Thermo Fisher Scientific Center for Multiplexed Proteomics (TCMP) is a multiplex quantitative proteomic analysis core which started its operation in 2014 under the direction of Professor Steven P. Gygi of Department of Cell Biology at Harvard Medical School along and in partnership with Thermo Fisher Scientific. TCMP's mission is to provide multiplexed quantitative proteomic expression profiling to the Harvard Medical School community as well as external users.
Our center uses high resolution mass spectrometers and isobaric tandem mass tags (TMT) to quantify protein or phosphopeptide expression from cells, tissues or immunoprecipitated protein samples. Currently the TMT technology allows for the analysis of 6-16 samples within a single experiment by sample multiplexing. This allows us to study several conditions in a single experiment. To maximize quantitative accuracy and precision, synchronous precursor selection (SPS)-based MS3 technology is used, developed by Gygi Lab.
The facility utilizes an Orbitrap Eclipse™ Tribrid™ Mass Spectrometer coupled with a Liquid Chromatography System (EASY-nLC™ 1200 System) and a wet lab. TCMP uses in-house built software for peptide identification and quantification with custom data analysis software.