About Thermo Fisher Scientific Center for Multiplexed Proteomics

        Thermo Fisher Scientific Center for Multiplexed Proteomics (TCMP) is a multiplex quantitative proteomic analysis service, which started its operation in 2014 under the direction of Prof. Steven P Gygi of Department of Cell Biology, Harvard Medical School along with his Post-Doctoral Fellow Ryan K. Kunz in partnership with Thermo Fisher Scientific. TCMP`s mission is to provide  multiplexed quantitative protein expression profiling to the Harvard Medical School community as well as external users.

      The Center uses High Resolution Mass Spectrometers and Isobaric Tandem Mass Tags (TMT) to quantify protein or phosphopeptide expression from cells, tissues or immunoprecipitated protein samples. Currently the TMT technology allows for the analysis of 6 - 16 samples within a single experiment by sample multiplexing. This allows us to study several conditions in a single experiment. To maximize quantitative accuracy and precision, synchronous precursor selection (SPS)-based MS3 technology is used, developed by Gygi Lab.

      The facility utilizes an Orbitrap Eclipse™ Tribrid™ Mass Spectrometer  coupled with a Liquid Chromatography System (EASY-nLC™ 1200 System) and a wet lab.  TCMP uses in-house built software for peptide identification and quantification with custom data analysis software.