Rates

1. Total Proteome-wide analysis price includes protein lysate quantification, reduction & alkylation, precipitation and digestion followed by peptide quantification, TMT-labeling, LC-MS3 label check, basic reverse-phase HPLC fractionation (bRP-HPLC), LC-MS3 analysis of 12 bRP-LC peptide fractions(~36 hours of MS analysis), database searching, filtering to 1% FDR at protein level, TMT reporter quantification, data analysis and final report preparation. We typically quantify between 7,000-10,000 proteins based on the sample and protein quality.

• Note We prefer to have a minimum of 100 micrograms of protein from each sample for starting amounts for this analysis.

2. Mini Phospho analysis is offered as an economical add-on to Proteome-wide analysis. For Mini Phospho analysis(3 hours of MS analysis), additional cost to Service No.1 includes phosphopeptide enrichment, single LC-MS2 analysis of an enriched fraction. Data processing is similar to Service No. 1 and report will include the phosphorylated site localization and quantification.

• Note No further fractionation of phospho-enriched part is done. We have seen ~6000 sites in one-shot based sample quality.

3. Full Phosphoproteome analysis price includes protein lysate quantification, reduction & alkylation, precipitation and digestion followed by peptide quantification, phosphopeptide enrichment, TMT-labeling, mixing, LC-MS3 Label Check, bRP-HPLC fractionation, LC-MS2 analysis of 12 phosphopeptide fractions(~36 hours of MS analysis). Data processing is similar to Service No. 1 and report will include the phosphorylated sites from 12 fractions. We have typically seen ~15,000 sites.

• Note Higher starting amounts are required for this analysis. Minimum of 1-2 mg or more for each sample is required.

4. Phosphotyrosine (pY) analysis is offered as an add-on to Phosphoproteome analysis. For pY analysis, additional cost to Service No. 3 includes pY enrichment using pY1000 antibody from Cell Signaling Technology, single LC-MS2 analysis of pY enriched peptides. Data processing is similar to Service No. 1 and report will include the pY sites from single pY enriched fractions.

• Note No further fractionation of pY enriched part is done.
• Note Higher starting amounts are required for this analysis. Minimum of 5 mg or more for each sample is required.

5. Ubiquitin-ome analysis price includes protein lysate quantification, reduction & alkylation, precipitation and digestion followed by peptide quantification, di-glycine enrichment (ubiquitin remnant after digestion) using Cell Signaling Technology’s –GG antibody, TMT-labeling, LC-MS3 Label Check, bRP-HPLC, LC-MS3 analysis of 12 bRP-LC fractions. Data processing is similar to Service No. 1 and report will include the ubiquitinated sites from 12 fractions.

• Note Higher starting amounts are required for this analysis. Minimum of 5 mg or more for each sample is preferred.

6. Quantitative Immunoprecipitation (Quant-IP) analysis: Please review sample submission guidelines. Based on the nature and/or volume of submitted sample, we perform in-solution / on-Bead digestion, TMT-labelling, one 3 hour LC-MS3 analysis, database searching, filtering to a 1% FDR, quantification, data analysis and final report preparation.

• Note No orthogonal fractionation is done for Quant-IP.

7. New Service for 2021: Quantitative Immunoprecipitation (Quant-IP) with extensive fractionation analysis(18 hours): Please review sample submission guidelines. Based on the nature and/or volume of submitted sample, we perform in-solution / on-Bead / In-Gel digestion, TMT-labelling, basic reverse-phase HPLC fractionation using spin columns, six 3 hour LC-MS3 analysis, database searching, filtering to a 1% FDR, quantification, data analysis and final report preparation.

• Note Quantitative Immunoprecipitation (Quant-IP) with extensive fractionation analysis requires at least 20 micrograms of protein per sample.